Display Settings:

Format

Send to:

Choose Destination
We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Curr Opin Microbiol. 2010 Jun;13(3):263-73. doi: 10.1016/j.mib.2010.02.008. Epub 2010 Mar 19.

Engineering of regulatory cascades and networks controlling antibiotic biosynthesis in Streptomyces.

Author information

  • 1Area de Microbiología, Department of Molecular Biology, University of León, León, Spain. jf.martin@unileon.es

Abstract

Engineering of regulatory mechanisms that control the biosynthesis of bioactive secondary metabolites is an approach to increase the production of valuable fermentation products. Two types of regulatory mechanisms have been studied in Streptomyces species: (1) pyramidal cascades of regulation that usually involve a butyrolactone and its receptor protein triggering the formation of pathway-associated regulatory proteins (SARP), and (2) global regulators that transduce protein phosphorylation signals responding to stress factors. Global regulators are frequently two-component systems; for example, the PhoR-PhoP system, the AsbA1-AsbA2, the orphan response regulator GlnR and the STAND-family regulator AfsR. Several strategies have been used to obtain overproducer strains, including: (i) obtention of phosphate-deregulated mutants by alteration of phoP, (ii) amplification and/or overexpression of pathway-associated positive regulators, and (iii) modification of butyrolactone receptor proteins. The success of these strategies is hampered by the poor knowledge of interactions between regulatory mechanisms.

Copyright 2010 Elsevier Ltd. All rights reserved.

PMID:
20303823
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk