(A-C) Wings from wt female flies (A), female flies expressing dJub RNAi (B), or female flies expressing dJub-mCherry transgene (C). 1096-Gal4 was used to drive RNAi or transgene expression. (D) Outlines of the wings in panels A-C. (E, F) Quantification of relative wing areas (E) and cell numbers (F) of genotypes in A-C. Area and cell number measurements were taken from the wing region located between veins L4 and L5, and wt defined as 100% (N=20 for each). (G) Extracts of mammalian HEK293 cells transfected with myc-dJub immunoblotted with dJub antiserum (left) or Myc antiserum (right). (H) Immunoblot analysis of dJub protein levels in wt or dJub RNAi-expressing larval eye imaginal discs. Actin serves as loading control. Relative amount of dJub protein is indicated below each lane. Mid-pupal wt eyes (I) or GMR-Gal4 driven dJub RNAi expressing eyes (J) stained for DE-cadherin. Secondary (arrows) and tertiary (arrowheads) interommatidial cells are highlighted. Loss of interommatidial cells in dJub RNAi expressing pupal eyes denoted by arrows (J). (K) Quantification of relative numbers of interommatidial cells in wt versus dJub RNAi pupal eye. Interommatidial cells were counted in 20 fields, each containing a cluster of at least 7 ommatidia. (L-O) Mid-pupal wt eyes stained for DE-cadherin (L), dJub (M), and merged image (N). Z-stack analysis of line in N is shown above panel N (N). (O) Immunostaining with dJub antiserum preabsorbed with immunizing peptide. (P-R) Scanning electron micrographs (SEMs) of female adult eyes. WT (Q), djub^I generated via the EGUF-Hid method, which results in eyes composed almost entirely of mutant tissue (Q), and GMR-gal4 driven overexpression of UAS-dJub-mCherry transgene (R). (S-U) Female third-instar larval eye imaginal discs containing wt (S) or djub^I mutant (T, U) clones marked by the absence of GFP expression (black). (U) Enlarged view of djub^I and wt twin spot clones. Yellow arrow identifies djub^I clones, red arrow identifies wt twin spot clone containing two copies of Ubi-GFP, and white arrow identifies tissue carrying one copy of Ubi-GFP. In all experiments wings and eyes were dissected from female flies. In graphs data are shown as mean percentages +/− standard deviation, with N= 20 for each genotype. (***) Represents p-value ≤ 0.001 and (*) represents p-value ≤ 0.05. Anterior is to the left for all larval imaginal discs.

(A-H) Female mid-pupal eyes stained for DE-cadherin (red). Wt, GFP positive MARCM clones (A); djub^I MARCM clones (GFP +ve) (B); MARCM clones overexpressing Yki (GFP +ve) (C); MARCM clones mutant for djub^I and overexpressing Yki (GFP +ve) (D); MARCM clones expressing wts RNAi (GFP +ve) (E); MARCM clones mutant for djub^I and expressing wts RNAi (GFP +ve) (F); MARCM clones expressing hpo RNAi (GFP +ve) (G); MARCM clones mutant for djub^I and expressing hpo RNAi (GFP +ve) (H). (I) Quantification of the clonal area (GFP +ve) for each genotype as a percentage of the entire pupal eye area. (J) Quantification of the percent increase of interommatidial cells within the clonal area (GFP +ve) as compared to wild type (set at 100% IOCs) for each genotype. In graphs data are shown as mean percentages +/− standard deviation, with N= 10 for each genotype. (***) Represents p-value ≤ 0.001. Scale bars in (A-H) equal 20μm.

(A-C) Female third-instar larval eye imaginal discs containing djub^I clones (GFP –ve, yellow arrows) generated by Eyeless-flp and stained for DIAP1 (A’), DIAP1-lacZ (B’), or Cyclin E (C’) expression. Anterior is to the left for all larval eye imaginal discs. (D-K) SEMs of adult female Drosophila eyes of wt (E) and Hippo pathway mutants (F,H,J) and djub^I (E) or Hippo pathway mutants containing a deletion of a single copy of djub (G,I,K), as indicated. Mid-pupal eye dissection of Hippo pathway mutants (L,N,P) or Hippo pathway mutants containing a deletion of a single copy of djub (M,O,Q), as indicated, and stained for DE-cadherin to identify interommatidial cells. Scale bars in (D-K) equal 100μm and (L-Q) equal 10μm. (R) Quantification of interommatidial cell numbers in 10 random fields containing 10 ommatidia each of genotypes (D,F-K). Data are shown as mean percentages +/− standard deviation and (***) represents p-value ≤ 0.001.

(A-D) HEK293 cells were co-transfected with LIMD1-YFP (A), Ajuba-YFP (B), Myc-WTIP (C), or Myc-Zyxin (D) and Flag-tagged Mst1, Lats1/2, WW45 or YAP, as indicated. Cell lysates were immunoprecipitated for each Hippo pathway member (anti-Flag), and bound products Immunoblotted (IB) for the presence of each LIM protein (anti-YFP or anti-Myc). Immunoblots of input controls (5%) are shown on the right side of each panel. (E) HEK293 cells were transfected with the indicated member of the Hippo pathway in the absence or presence of LIMD1-YFP. Levels of phospho-S127-YAP (upper panel) or total YAP (lower panels) were then determined by immunoblot (IB) analysis. Relative amounts of phospho-S127-YAP with respect to total YAP protein is indicated below each lane. (F) MDCK cells were transfected with control Luc siRNA (lanes 1-3) or Ajuba and LIMD1 siRNAs (lanes 4-6) and then plated at low (LD), medium (MD) and high density (HD). Amount of S127-YAP phosphorylation relative to total YAP for each density within control and Ajuba/LIMD1 depleted cells was determined by immunoblotting. The relative level of YAP phosphorylation for each density between control and Ajuba/LIMD1 depleted cells was determined and is indicated above the lanes. (G) Working model, based upon results herein, for how Ajuba LIM proteins could influence Hippo pathway signaling.