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J Endocrinol. 1991 Apr;129(1):131-9.

Concentrations of arachidonate metabolites, steroids and histamine in preovulatory horse follicles after administration of human chorionic gonadotrophin and the effect of intrafollicular injection of indomethacin.

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  • 1Section of Reproductive Studies, University of Pennsylvania, School of Veterinary Medicine, Kennett Square, Pennsylvania 19348.


This study investigated the sequence of hormonal changes within the preovulatory follicles of mares. Mares were injected i.v. with 2500 IU human chorionic gonadotrophin (hCG) when a preovulatory follicle of 35 mm in diameter was detected. Fluid was aspirated from preovulatory follicles before (0 h), and 12, 24 and 36 h after administration of hCG. Concentrations of progesterone, prostaglandin (PG) E2, PGF, 6-keto-PGF1 alpha and thromboxane B2 in follicular fluid increased significantly (P less than 0.01) between 0 and 36 h. At 36 h, PGE2 was present in highest concentrations, followed by PGF and 6-keto-PGF1 alpha; thromboxane B2 was present at lower concentrations than other prostanoids. Concentrations of 13,14-dihydro-15-keto-PGF2 alpha increased significantly (P less than 0.05) between 24 and 36 h. Leukotriene B4, leukotriene C4 and histamine were present in follicular fluid at all sampling periods and did not change significantly over time. In another experiment, buffered saline or indomethacin (either 100 or 500 micrograms) was injected into preovulatory follicles on the day that they reached 35 mm in diameter to determine whether blocking intrafollicular PG synthesis would affect ovulation. The interval between intrafollicular injection and ultrasonographic detection of luteinization was significantly longer (P less than 0.05) in mares treated with 500 micrograms indomethacin. Plasma progesterone concentrations were significantly (P less than 0.05) lower in indomethacin-treated mares than in control mares on the first 5 days after injection. These results indicate that intrafollicular concentrations of PGs increase significantly before ovulation in mares and may be involved in the ovulatory process.

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