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Nat Chem Biol. 2010 Apr;6(4):273-5. doi: 10.1038/nchembio.317. Epub 2010 Mar 14.

Dioxygenases catalyze the O-demethylation steps of morphine biosynthesis in opium poppy.

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  • 1Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada.

Abstract

Two previously undetected enzymes involved in morphine biosynthesis and unique among plants to opium poppy have been identified as non-heme dioxygenases, in contrast to the functionally analogous cytochrome P450s found in mammals. We used functional genomics to isolate thebaine 6-O-demethylase (T6ODM) and codeine O-demethylase (CODM), the only known 2-oxoglutarate/Fe(II)-dependent dioxygenases that catalyze O-demethylation. Virus-induced gene silencing of T6ODM and CODM in opium poppy efficiently blocked metabolism at thebaine and codeine, respectively.

PMID:
20228795
[PubMed - indexed for MEDLINE]
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