Triad 1 induces apoptosis by p53 activation

FEBS Lett. 2010 Apr 16;584(8):1565-70. doi: 10.1016/j.febslet.2010.03.011. Epub 2010 Mar 10.

Abstract

Triad 1 (2 RING [really interesting new gene] fingers and DRIL [double RING finger linked] 1) is an E3 ligase that induces apoptosis and clonogenic inhibition in myeloid cells through Gfi-1 stabilization. Here we demonstrate that Triad 1 induces apoptosis in several cancer cell lines including MCF7, A549, U2OS, and HCT 116 p53(+/+) cells via its RING ligase activity. Interestingly, in these cancer cells, Triad 1-induced apoptosis is not mediated by Gfi-1 stabilization but is instead p53-dependent. Moreover, Triad 1 promotes transactivation of p53. These results suggest that Triad 1 can induce apoptosis through its ligase activity via p53 activation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis*
  • Cell Line, Tumor
  • Cell Proliferation
  • Humans
  • RING Finger Domains*
  • Transcription Factors / metabolism
  • Tumor Suppressor Protein p53 / metabolism*
  • Ubiquitin-Protein Ligases / chemistry
  • Ubiquitin-Protein Ligases / metabolism*

Substances

  • Transcription Factors
  • Tumor Suppressor Protein p53
  • Ubiquitin-Protein Ligases