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Nucleic Acids Res. 2010 Jul;38(13):4466-75. doi: 10.1093/nar/gkq160. Epub 2010 Mar 11.

Efficient inhibition of miR-155 function in vivo by peptide nucleic acids.

Author information

  • 1Medical Research Council, Laboratory of Molecular Biology, Cambridge CB2 0QH, UK. mfabani@mrc-lmb.cam.ac.uk

Abstract

MicroRNAs (miRNAs) play an important role in diverse physiological processes and are potential therapeutic agents. Synthetic oligonucleotides (ONs) of different chemistries have proven successful for blocking miRNA expression. However, their specificity and efficiency have not been fully evaluated. Here, we show that peptide nucleic acids (PNAs) efficiently block a key inducible miRNA expressed in the haematopoietic system, miR-155, in cultured B cells as well as in mice. Remarkably, miR-155 inhibition by PNA in primary B cells was achieved in the absence of any transfection agent. In mice, the high efficiency of the treatment was demonstrated by a strong overlap in global gene expression between B cells isolated from anti-miR-155 PNA-treated and miR-155-deficient mice. Interestingly, PNA also induced additional changes in gene expression. Our analysis provides a useful platform to aid the design of efficient and specific anti-miRNA ONs for in vivo use.

PMID:
20223773
[PubMed - indexed for MEDLINE]
PMCID:
PMC2910044
Free PMC Article

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