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    Zh Mikrobiol Epidemiol Immunobiol. 2010 Jan-Feb;(1):20-3.

    [Enzyme immunoassay-based analysis of OspC recombinant proteins from Borrelia garinii and Borrelia afzelii isolated in West Siberia].

    [Article in Russian]

    Abstract

    AIM:

    To study the ability of OspC recombinant proteins from Borrelia garinii and Borrelia afzelii isolated in West Siberia to interact with serum antibodies from patients with tick-borne borreliosis (TBB).

    MATERIALS AND METHODS:

    Recombinant antigens OspC B. garinii and OspC B. afrelii, serum samples from patients with TBB were used as well as solid-phase enzyme immunoassay and parametric and non-parametric statistical methods.

    RESULTS:

    Higher antigenic activity of B. garinii OspC compared with OspC from B. afzelii was observed when these recombinant proteins were compared in enzyme immunoassay. Detection rate of class M and G immunoglobulins to B. garinii OspC in sera of patients with TBB was 60.5% and 70% respectively.

    CONCLUSION:

    Obtained results indicate high immunoreactivity of OspC recombinant proteins from B. garinii and B. afzelii and point to perspective of their combined use for serological diagnostics of TBB.

    PMID:
    20218339
    [PubMed - indexed for MEDLINE]

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