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Chromosome Res. 2010 Apr;18(3):337-47. doi: 10.1007/s10577-010-9119-x. Epub 2010 Mar 5.

Functional centromeres in soybean include two distinct tandem repeats and a retrotransposon.

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  • 1Research Institute for Bioresources, Okayama University, Chuo 2-20-1, Kurashiki, 710-0046, Japan. altek2@gmail.com

Abstract

The centromere as a kinetochore assembly site is fundamental to the partitioning of genetic material during cell division. In order to determine the functional centromeres of soybean, we characterized the soybean centromere-specific histone H3 (GmCENH3) protein and developed an antibody against the N-terminal end. Using this antibody, we cloned centromere-associated DNA sequences by chromatin immunoprecipitation. Our analyses indicate that soybean centromeres are composed of two distinct satellite repeats (GmCent-1 and GmCent-4) and retrotransposon-related sequences (GmCR). The possible allopolyploid origin of the soybean genome is discussed in view of the centromeric satellite sequences present.

PMID:
20204495
[PubMed - indexed for MEDLINE]
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