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J Biol Chem. 2010 Apr 23;285(17):12469-73. doi: 10.1074/jbc.C109.094599. Epub 2010 Mar 4.

Characterization and structure determination of the Cdt1 binding domain of human minichromosome maintenance (Mcm) 6.

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  • 1Department of Physics and Shanghai Key Laboratory for Magnetic Resonance, East China Normal University, Shanghai 200062, China.

Abstract

The minichromosome maintenance (Mcm) 2-7 complex is the replicative helicase in eukaryotic species, and it plays essential roles in the initiation and elongation phases of DNA replication. During late M and early G(1), the Mcm2-7 complex is loaded onto chromatin to form prereplicative complex in a Cdt1-dependent manner. However, the detailed molecular mechanism of this loading process is still elusive. In this study, we demonstrate that the previously uncharacterized C-terminal domain of human Mcm6 is the Cdt1 binding domain (CBD) and present its high resolution NMR structure. The structure of CBD exhibits a typical "winged helix" fold that is generally involved in protein-nucleic acid interaction. Nevertheless, the CBD failed to interact with DNA in our studies, indicating that it is specific for protein-protein interaction. The CBD-Cdt1 interaction involves the helix-turn-helix motif of CBD. The results reported here provide insight into the molecular mechanism of Mcm2-7 chromatin loading and prereplicative complex assembly.

PMID:
20202939
[PubMed - indexed for MEDLINE]
PMCID:
PMC2857124
Free PMC Article
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