Agronomic use of biosolids has raised concern that plant availability of biosolids-Cd will increase with time after cessation of biosolids application. It has been demonstrated that chemical extractability of Cd is persistently decreased in biosolids-amended soils. This study was conducted to determine if Cd phytoavailability in long-term biosolids-amended soils was also persistently decreased. Paired control and biosolids-amended soils were collected from three experimental sites where large cumulative rates of biosolids were applied about 20 yr ago. The pH of all soils [in 0.01 mol L(-1) Ca(NO(3))(2)] was adjusted to 6.5 +/- 0.2. Increasing rates of Cd-nitrate (from 0 to 10.0 mg Cd kg(-1) soil) enriched in (111)Cd stable isotope were added to all soils, and Romaine lettuce (Lactuca sativa L. var. longifolia Lam.) was grown in pots to bioassay phytoavailable Cd. After harvest, Cd concentrations in shoots and labile pool of Cd (Cd(L)) in soils were determined. The relationship between added salt-Cd and Cd concentrations in lettuce shoots was linear for all soils tested. Ratios of (shoot Cd):(soil Cd) slopes were highest in the control soils. Biosolids amendment decreased (shoot Cd):(soil Cd) slopes to varied extent depending on biosolids source, properties, and application rate. The decrease in slope in comparison to the control was an indication of the lower phytoavailability of Cd in biosolids-amended soils. A significant negative correlation existed between Cd uptake slopes and soil organic matter, free and amorphous Fe and Al oxides, Bray-P, and soil and plant Zn. Biosolids-Cd was highly labile (%L 80-95) except for Fulton County soil (%L = 61).