Format

Send to:

Choose Destination
See comment in PubMed Commons below
Opt Express. 2010 Jan 18;18(2):877-84. doi: 10.1364/OE.18.000877.

Multiplane imaging and three dimensional nanoscale particle tracking in biological microscopy.

Author information

  • 1School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh, UK. P.A.Dalgarno@hw.ac.uk

Abstract

A conventional microscope produces a sharp image from just a single object-plane. This is often a limitation, notably in cell biology. We present a microscope attachment which records sharp images from several object-planes simultaneously. The key concept is to introduce a distorted diffraction grating into the optical system, establishing an order-dependent focussing power in order to generate several images, each arising from a different object-plane. We exploit this multiplane imaging not just for bio-imaging but also for nano-particle tracking, achieving approximately 10 nm z position resolution by parameterising the images with an image sharpness metric.

PMID:
20173908
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Optical Society of America
    Loading ...
    Write to the Help Desk