Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Virology. 2010 May 10;400(2):157-63. doi: 10.1016/j.virol.2010.01.023. Epub 2010 Feb 20.

Deep sequencing analysis of viruses infecting grapevines: Virome of a vineyard.

Author information

  • 1Department of Genetics, Stellenbosch University, Matieland, 7602, South Africa.

Abstract

Double stranded RNA, isolated from 44 pooled randomly selected vines from a diseased South African vineyard, has been used in a deep sequencing analysis to build a census of the viral population. The dsRNA was sequenced in an unbiased manner using the sequencing-by-synthesis technology offered by the Illumina Genome Analyzer II and yielded 837 megabases of metagenomic sequence data. Four known viral pathogens were identified. It was found that Grapevine leafroll-associated virus 3 (GLRaV-3) is the most prevalent species, constituting 59% of the total reads, followed by Grapevine rupestris stem pitting-associated virus and Grapevine virus A. Grapevine virus E, a virus not previously reported in South African vineyards, was identified in the census. Viruses not previously identified in grapevine were also detected. The second most prevalent virus detected was a member of the Chrysoviridae family similar to Penicillium chrysogenum virus. Sequences aligning to two other mycoviruses were also detected.

Copyright 2010 Elsevier Inc. All rights reserved.

PMID:
20172578
[PubMed - indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk