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Mol Cell Endocrinol. 2010 May 14;320(1-2):45-50. doi: 10.1016/j.mce.2010.02.009. Epub 2010 Feb 18.

Melanin-concentrating hormone facilitates migration of preadipocytes.

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  • 1Department of Biology, The College at Brockport, State University of New York, 350 New Campus Drive, Brockport, NY 14420, USA. lcook@brockport.edu

Abstract

Adipose tissue develops from differentiating preadipocytes that expand and migrate. 3T3-L1 preadipocytes respond to melanin-concentrating hormone (MCH) by increasing leptin production. Here, we investigate whether MCH elicits remodeling of the actin cytoskeleton and whether this translates into altered migratory capacity of these cells. Incubation with MCH resulted in a loss of actin stress fibers accompanied by a change in morphology from a stretched-out fibroblast to a rounded cell. PMC-3881-PI, a MCH receptor 1 antagonist blocked the effect, confirming this receptor is solely responsible for MCH-mediated actin rearrangements. Both a pharmacological activator and inhibitor of phospholipase C were used to demonstrate this molecule's importance to the signaling pathway. Finally, MCH was shown to facilitate preadipocyte migration into a scratch wound, revealing a previously unknown role for MCH in the regulation of cellular migration. We conclude that MCH could influence the expansion of adipose tissue through its ability to enhance preadipocyte migration.

(c) 2010 Elsevier Ireland Ltd. All rights reserved.

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