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Genes Chromosomes Cancer. 2010 May;49(5):471-9. doi: 10.1002/gcc.20757.

Atypical mRNA fusions in PML-RARA positive, RARA-PML negative acute promyelocytic leukemia.

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  • 1Pathologisches Institut, Universit√§tsmedizin Mannheim, Theodor-Kutzer-Ufer 1-3, Mannheim, Germany.

Abstract

Reciprocal RARA-PML transcripts are not detected in approximately 25% of patients with PML-RARA positive acute promyelocytic leukemia (APL), but the reasons for this are poorly understood. We studied 21 PML-RARA positive/RARA-PML negative cases by bubble PCR and multiplex long template PCR to identify the genomic breakpoints. Additional RT-PCR analysis was performed based on the DNA findings. Three cases were found to have complex rearrangements involving a third locus: the first had a PML-CDC6-RARA forward DNA fusion and expressed a chimeric PML-CDC6-RARA mRNA in addition to a PML-RARA. The other two had HERC1-PML and NT_009714.17-PML genomic fusion sequences at their respective reciprocal breakpoints. Six patients were falsely classified as RARA-PML negative due to deletions on chromosome 15 and/or 17, or alternative splicing leading to atypical RARA-PML fusion transcripts, which were not identified by conventional RT-PCR assays. This study demonstrates that the frequency of RARA-PML expression has been underestimated and highlights remarkable complexity at chromosomal breakpoint regions in APL even in cases with an apparently simple balanced t(15;17)(q24;q12).

(c) 2010 Wiley-Liss, Inc.

PMID:
20155840
[PubMed - indexed for MEDLINE]
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