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J Trauma. 2010 Feb;68(2):434-40. doi: 10.1097/TA.0b013e31819adb38.

Effect of Astragalus membranaceus in rats on peripheral nerve regeneration: in vitro and in vivo studies.

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  • 1School of Post Baccalaureate Chinese Medicine, Changhua Christian Hospital, Changhua. lumc@mail.cmu.edu.tw

Abstract

BACKGROUND:

This study provides in vitro and in vivo evaluation of Astragalus membranaceus (AM) on peripheral nerve regeneration.

METHODS:

In the in vitro study, we analyzed the effects of AM on cell differentiation and neurite outgrowth by using a PC12 cell model. In the in vivo study, silicone rubber chambers filled with the AM water extract were used to bridge a 10-mm sciatic nerve defect in rats.

RESULTS:

We found that the AM water extract caused a marked enhancement of the nerve growth factor-mediated neurite outgrowth and the expression of growth-associated protein 43 from PC12 cells. Animals from the groups treated with the AM for 8 weeks had a relatively more mature structure with larger mean values of myelinated axon number, endoneurial area, and total nerve area when compared with those in the controls receiving the saline only.

CONCLUSION:

These results suggest that the silicone rubber tubes present a good framework for the nerve fibers to regenerate across the gap, and the AM extract can be a potential nerve growth-promoting factor, being salutary in aiding the growth of axons in the peripheral nerve.

PMID:
20154555
[PubMed - indexed for MEDLINE]
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