Send to

Choose Destination
See comment in PubMed Commons below
Mol Cell Biol. 2010 Apr;30(8):1937-45. doi: 10.1128/MCB.01370-09. Epub 2010 Feb 12.

Uncoupling of expression of an intronic microRNA and its myosin host gene by exon skipping.

Author information

  • 1Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA.


The ancient MYH7b gene, expressed in striated muscle and brain, encodes a sarcomeric myosin and the intronic microRNA miR-499. We find that skipping of an exon introduces a premature termination codon in the transcript that downregulates MYH7b protein production without affecting microRNA expression. Among other genes, endogenous miR-499 targets the 3' untranslated region of the transcription factor Sox6, which in turn acts as a repressor of MYH7b transcriptional activity. Thus, concerted transcription and alternative splicing uncouple the level of expression of MYH7b and miR-499 when their coexpression is not required.

[PubMed - indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk