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Mol Cell Biol. 2010 Apr;30(8):1937-45. doi: 10.1128/MCB.01370-09. Epub 2010 Feb 12.

Uncoupling of expression of an intronic microRNA and its myosin host gene by exon skipping.

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  • 1Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA.

Abstract

The ancient MYH7b gene, expressed in striated muscle and brain, encodes a sarcomeric myosin and the intronic microRNA miR-499. We find that skipping of an exon introduces a premature termination codon in the transcript that downregulates MYH7b protein production without affecting microRNA expression. Among other genes, endogenous miR-499 targets the 3' untranslated region of the transcription factor Sox6, which in turn acts as a repressor of MYH7b transcriptional activity. Thus, concerted transcription and alternative splicing uncouple the level of expression of MYH7b and miR-499 when their coexpression is not required.

PMID:
20154144
[PubMed - indexed for MEDLINE]
PMCID:
PMC2849460
Free PMC Article
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