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Fertil Steril. 2010 Oct;94(5):1616-21. doi: 10.1016/j.fertnstert.2009.10.038. Epub 2010 Feb 13.

Evaluation of erythrocyte band 3 phosphotyrosine level, glutathione content, CA-125, and human epididymal secretory protein E4 as combined parameters in endometriosis.

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  • 1Department of Biological Chemistry, University of Padova, Padova, Italy.

Abstract

OBJECTIVE:

To investigate the biochemical parameters of the erythrocyte response to diamide-induced oxidative stress, alone or as adjuncts to serum values of CA-125 and human epididymal secretory protein E4 (HE4), in the diagnosis and study of endometriosis.

SETTING:

University of Padova.

DESIGN:

Prospective study.

PATIENT(S):

Forty-five patients of reproductive age undergoing laparoscopy.

INTERVENTION(S):

All women were studied for endometriotic foci during laparoscopic surgery. Forty-one had laparoscopically and histologically confirmed endometriosis, and four did not. Twenty women with confirmed endometriosis were reassessed 1-4 months later.

MAIN OUTCOME MEASURE(S):

CA-125 and HE4 and two new parameters evaluated in erythrocytes after diamide-induced stress, that is, band 3 tyrosine phosphorylation (Tyr-P) level and decrease in total glutathione content (ΔGSH), were assessed in all patients.

RESULT(S):

In association with serum CA-125 levels but not with HE4, diamide-related erythrocyte band 3 Tyr-P and ΔGSH were significantly higher in patients with endometriosis and were able to discriminate with high sensitivity and specificity between patients before and after surgery.

CONCLUSION(S):

Endometriosis is associated with an increase in systemic oxidative stress, affecting the antioxidative defenses of circulating erythrocytes. All related implications, including evaluation of other oxidative stress-related changes, warrant further study.

Copyright © 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

PMID:
20153468
[PubMed - indexed for MEDLINE]
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