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Mol Biotechnol. 2010 Jun;45(2):180-6. doi: 10.1007/s12033-010-9251-z.

Cell-based assays for high-throughput screening.

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  • 1Chemical Biology Platform, The Broad Institute, 7 Cambridge Center, Cambridge, MA 02142, USA. fan@broadinstitute.org

Abstract

Cell-based assays represent approximately half of all high-throughput screens currently performed. Here, we review in brief the history and status of high-throughput screening (HTS), and summarize some of the challenges and benefits associated with the use of cell-based assays in HTS. Approaches for successful experimental design and execution of cell-based screens are introduced, including strategies for assay development, implementation of primary and secondary screens, and target identification. In doing so, we hope to provide a comprehensive review of the cell-based HTS process and an introduction to the methodologies and techniques used.

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