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Cold Spring Harb Protoc. 2009 Oct;2009(10):pdb.prot5310. doi: 10.1101/pdb.prot5310.

Virgin collection and haplodiploid crossing methods in Nasonia (parasitoid wasp).

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  • 1Department of Biology, University of Rochester, Rochester, NY 14627, USA. werr@mail.rochester.edu


This protocol covers most procedures used by the Nasonia (wasp) geneticist, including virgin collection. Nasonia are easily sexed as pupae, so collecting virgins can easily be performed within a window of several days. The female-specific ovipositor is visible at even the earliest pupal stages as a longitudinal ridge on the ventral posterior abdomen. To prolong generations, yellow pupae can be stored at 4 degrees C-8 degrees C for up to 2 mo and adults for 2-3 wk. In the example presented here, haplodiploid crossing methods are used to make a new Nasonia vitripennis mutant homozygous, using an aunt/nephew mating approach. The protocol can be extended to inbreeding strains. Inbreeding depression generally is not a problem in Nasonia unless the mutation itself is female-sterile. Mother/son mating and sib mating are also reasonable approaches to inbreeding.

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