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Cell. 2010 Feb 5;140(3):313-26. doi: 10.1016/j.cell.2010.01.028.

Methods in mammalian autophagy research.

Author information

  • 1Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo 113-8519, Japan. nmizu.phy2@tmd.ac.jp

Abstract

Autophagy has been implicated in many physiological and pathological processes. Accordingly, there is a growing scientific need to accurately identify, quantify, and manipulate the process of autophagy. However, as autophagy involves dynamic and complicated processes, it is often analyzed incorrectly. In this Primer, we discuss methods to monitor autophagy and to modulate autophagic activity, with a primary focus on mammalian macroautophagy.

2010 Elsevier Inc. All rights reserved.

PMID:
20144757
[PubMed - indexed for MEDLINE]
PMCID:
PMC2852113
Free PMC Article

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