Validation of the 3D profile method for prediction of fibrillizing segments. The top and bottom panels show the predicted energy for fibrillation of every six-residue segment of RNase A. Red histogram bars represent hexapeptides with energy below -23 kcal/mol and are predicted to form fibrils. Seven such segments are shown, and each forms fibrils, as shown by the electron micrographs in the central panels. The black arrows indicate where three of these segments lie in the 3D structure of RNase A. This cartoon structure, like the histogram of energies, is colored with warmer colors representing a greater propensity for fibrillation. Blue and green segments are of higher energy and are predicted not to form fibrils. FERQHM is one of several segments predicted and experimentally confirmed not to form fibrils (first row of micrographs, right). However, when the residues of this segment were rearranged to QEMRHF (Table S2), the energy of the rearranged segment falls below -23 kcal/mol; QEMRHF is thus predicted to form fibrils, and it does (first row of micrographs). In addition, the fibril-forming segments QANKHI and STMSIT were rearranged to IHKAQN and ISMTTS, respectively, two sequences predicted and confirmed not to form fibrils (second and third row of micrographs). Notice that the longer SSTSAASSNY segment contains the SSTSAA segment, which forms fibrils and is capable of forming a steric zipper (Sawaya 2007). Taken together, the fibrillizing behavior of these 10 segments suggests that a threshold of -23 kcal/mol is appropriate for predicting fibrillation. The * indicates the C-terminal hinge loop, discussed in the text.

Sequence is more important than residue composition in determining propensity for formation of amyloid-like fibrils. (A) shows on the y axis the fibrillation propensity, calculated by the 3D profile method, and on the x axis the solvent exposure of segments in the nonredundant structures (proteins having less than 50% sequence identity) in the PDB. Segments below the horizontal -23 kcal/mol line are HP and are preferentially buried if < 15% exposed (vertical line). Thus buried HP segments are plotted in the lower left quadrant and are enclosed by a red rectangle. In (B) these segments are shuffled, and the fibrillation propensity and exposure of the resulting shuffled segments are plotted. The shuffling operation, which changes the residue order within a segment but does not alter its residue composition, allows these segments to migrate from the lower left quadrant to lower values of propensity (higher energy) and greater exposure to solvent. Only 42% of segments remain buried and retain their high fibrillogenic propensity. The exposure to solvent of these segments is estimated by examining the corresponding positions within proteins that contain the exact residue sequence. Of the 720 permutations for a given six-residue segment, about 12 are found in some PDB protein, and hence the solvent exposure can be determined and plotted. Warmer colors indicate higher count density. The percentage of segments found in each quadrant is indicated in white. The implications are that high propensity depends on a nonrandom pattern of sequences and that proteins tend to bury HP segments.