Format

Send to:

Choose Destination
See comment in PubMed Commons below
Nat Rev Genet. 2010 Mar;11(3):191-203. doi: 10.1038/nrg2732.

Principles and challenges of genomewide DNA methylation analysis.

Author information

  • USC Epigenome Center, University of Southern California, Keck School of Medicine, Los Angeles, 90089‑9601, USA. plaird@usc.edu

Abstract

Methylation of cytosine bases in DNA provides a layer of epigenetic control in many eukaryotes that has important implications for normal biology and disease. Therefore, profiling DNA methylation across the genome is vital to understanding the influence of epigenetics. There has been a revolution in DNA methylation analysis technology over the past decade: analyses that previously were restricted to specific loci can now be performed on a genome-scale and entire methylomes can be characterized at single-base-pair resolution. However, there is such a diversity of DNA methylation profiling techniques that it can be challenging to select one. This Review discusses the different approaches and their relative merits and introduces considerations for data analysis.

PMID:
20125086
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group
    Loading ...
    Write to the Help Desk