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Genes Dev. 2010 Feb 1;24(3):277-89. doi: 10.1101/gad.551810.

The genome-wide dynamics of the binding of Ldb1 complexes during erythroid differentiation.

Author information

  • 1Department of Cell Biology, Erasmus Medical Center, 3015GE Rotterdam, The Netherlands. e.soler@erasmusmc.nl

Erratum in

  • Genes Dev. 2010 Mar 15;24(6):623.

Abstract

One of the complexes formed by the hematopoietic transcription factor Gata1 is a complex with the Ldb1 (LIM domain-binding protein 1) and Tal1 proteins. It is known to be important for the development and differentiation of the erythroid cell lineage and is thought to be implicated in long-range interactions. Here, the dynamics of the composition of the complex-in particular, the binding of the negative regulators Eto2 and Mtgr1-are studied, in the context of their genome-wide targets. This shows that the complex acts almost exclusively as an activator, binding a very specific combination of sequences, with a positioning relative to transcription start site, depending on the type of the core promoter. The activation is accompanied by a net decrease in the relative binding of Eto2 and Mtgr1. A Chromosome Conformation Capture sequencing (3C-seq) assay also shows that the binding of the Ldb1 complex marks genomic interaction sites in vivo. This establishes the Ldb1 complex as a positive regulator of the final steps of erythroid differentiation that acts through the shedding of negative regulators and the active interaction between regulatory sequences.

PMID:
20123907
[PubMed - indexed for MEDLINE]
PMCID:
PMC2811829
Free PMC Article
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