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Methods. 2010 May;51(1):101-5. doi: 10.1016/j.ymeth.2010.01.031. Epub 2010 Feb 1.

Preparation of a highly active cell-free translation system from immature Xenopus laevis oocytes.

Author information

  • 1Department of Molecular Biology, Cell Biology & Biochemistry, Brown University, Providence, RI 02912, USA.

Abstract

Understanding mechanisms of post-transcriptional control of gene expression has come under much scrutiny in recent years. A key question in this field is how the translation of specific mRNAs is activated or repressed both spatially and temporally in a given cell. In oocytes of the frog Xenopus laevis a number of mRNAs are localized early in oogenesis and subsequently translated at later stages. We have developed a highly active cell-free translation system from oocytes in the early stages of oogenesis that is applicable to the study of translation and translational control of both endogenous and exogenous mRNAs.

PMID:
20123127
[PubMed - indexed for MEDLINE]
PMCID:
PMC2868112
Free PMC Article

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