Electrophoresis. 2010 Jan;31(3):448-58.

Development of a capillary gel electrophoresis method for monitoring disulfide isomer heterogeneity in IgG2 antibodies.

Lacher NA, Wang Q, Roberts RK, Holovics HJ, Aykent S, Schlittler MR, Thompson MR, Demarest CW.

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Analytical R&D, Pfizer BioTherapeutics R&D, Chesterfield, MO, USA. nathan.a.lacher@pfizer.com

Abstract

A CGE method for monitoring the disulfide isomer distribution characteristic of IgG2 MAbs is presented. Disulfide heterogeneity of MAbs has been studied using various chromatographic and electrophoretic methods. Although CGE operates using a different selectivity mechanism from that of sorption chromatographic techniques, similar trends are present in the data, which allow the CGE method to be used as a complementary method for studying disulfide isomer distribution. This article focuses on the optimization of a capillary-based gel electrophoresis method that can be used to support antibody development including bioprocess optimization, antibody characterization, release, and formulation stability assessment.

PMID:: 20119952; [PubMed - indexed for MEDLINE]

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