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Nat Methods. 2010 Feb;7(2):130-2. doi: 10.1038/nmeth.1417. Epub 2010 Jan 17.

FRT-seq: amplification-free, strand-specific transcriptome sequencing.

Author information

  • 1The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.

Abstract

We report an alternative approach to transcriptome sequencing for the Illumina Genome Analyzer, in which the reverse transcription reaction takes place on the flowcell. No amplification is performed during the library preparation, so PCR biases and duplicates are avoided, and because the template is poly(A)(+) RNA rather than cDNA, the resulting sequences are necessarily strand-specific. The method is compatible with paired- or single-end sequencing.

PMID:
20081834
[PubMed - indexed for MEDLINE]
PMCID:
PMC2861772
Free PMC Article

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