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Epigenetics. 2010 Jan 1;5(1):47-9. Epub 2010 Jan 8.

Accurate sodium bisulfite sequencing in plants.

Author information

  • 1Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge, UK.

Abstract

DNA cytosine methylation is a conserved epigenetic modification frequently correlating with transcriptional silencing in a wide variety of eukaryotic organisms. Sodium bisulfite treatment of DNA converts unmethylated cytosine to uracil, while 5-methylated cytosine is protected. We describe techniques that ensure reliable sequencing data following sodium bisulfite conversion and to avoid common pitfalls such as amplification of unconverted DNA and inclusion of sibling clones.

PMID:
20081358
[PubMed - indexed for MEDLINE]
PMCID:
PMC2829377
Free PMC Article

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