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Genes Dev. 2010 Jan 15;24(2):129-34. doi: 10.1101/gad.568610.

A novel, noncanonical mechanism of cytoplasmic polyadenylation operates in Drosophila embryogenesis.

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  • 1Gene Regulation Programme, Centre de Regulació Genòmica (CRG-UPF), 08003 Barcelona, Spain.

Abstract

Cytoplasmic polyadenylation is a widespread mechanism to regulate mRNA translation that requires two sequences in the 3' untranslated region (UTR) of vertebrate substrates: the polyadenylation hexanucleotide, and the cytoplasmic polyadenylation element (CPE). Using a cell-free Drosophila system, we show that these signals are not relevant for Toll polyadenylation but, instead, a "polyadenylation region" (PR) is necessary. Competition experiments indicate that PR-mediated polyadenylation is required for viability and is mechanistically distinct from the CPE/hexanucleotide-mediated process. These data indicate that Toll mRNA is polyadenylated by a noncanonical mechanism, and suggest that a novel machinery functions for cytoplasmic polyadenylation during Drosophila embryogenesis.

PMID:
20080951
[PubMed - indexed for MEDLINE]
PMCID:
PMC2807348
Free PMC Article
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