J Biol Chem. 1991 Apr 5;266(10):6015-8.

Analysis of the retention signals of two resident luminal endoplasmic reticulum proteins by in vitro mutagenesis.

Source

Department of Microbiology, St. Louis University School of Medicine, Missouri 63104.

Abstract

Protein disulfide isomerase (PDI, ERp59), ERp72, and ERp61 are luminal proteins of the endoplasmic reticulum (ER) that are characterized by the presence of sequences corresponding to the active site regions of PDI. Each one of these proteins possesses a different COOH-terminal tetrapeptide ER retention signal. In order to investigate what other tetrapeptide sequences could serve as retention signals and to determine to what extent the function of the retention signal is modulated by the protein carrying the signal, we have constructed a set of mutants of two of these resident ER proteins, PDI and ERp72. In each of these proteins, the wild type tetrapeptide sequences were replaced by each member of the set of the 12 possible combinations consisting of (K,R,Q)-(D,E)-(D,E)-L. Analysis of the efficiency of retention of the variant proteins when each was transiently expressed in COS cells showed that the retention efficiencies vary with both the COOH-terminal sequence and with the protein that carries this sequence.

PMID:: 2007562; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

HighWire Press - PDF

Supplemental Content

Save items

Related citations in PubMed

ERp72, an abundant luminal endoplasmic reticulum protein, contains three copies of the active site sequences of protein disulfide isomerase. [J Biol Chem. 1990]
ERp72, an abundant luminal endoplasmic reticulum protein, contains three copies of the active site sequences of protein disulfide isomerase.
Mazzarella RA, Srinivasan M, Haugejorden SM, Green M. J Biol Chem. 1990 Jan 15; 265(2):1094-101.
The stress response in Chinese hamster ovary cells. Regulation of ERp72 and protein disulfide isomerase expression and secretion. [J Biol Chem. 1990]
The stress response in Chinese hamster ovary cells. Regulation of ERp72 and protein disulfide isomerase expression and secretion.
Dorner AJ, Wasley LC, Raney P, Haugejorden S, Green M, Kaufman RJ. J Biol Chem. 1990 Dec 15; 265(35):22029-34.
Heavy chain binding protein (BiP/GRP78) and endoplasmin are exported from the endoplasmic reticulum in rat exocrine pancreatic cells, similar to protein disulfide-isomerase. [Arch Biochem Biophys. 1992]
Heavy chain binding protein (BiP/GRP78) and endoplasmin are exported from the endoplasmic reticulum in rat exocrine pancreatic cells, similar to protein disulfide-isomerase.
Takemoto H, Yoshimori T, Yamamoto A, Miyata Y, Yahara I, Inoue K, Tashiro Y. Arch Biochem Biophys. 1992 Jul; 296(1):129-36.
Review Protein disulfide isomerase: a multifunctional protein of the endoplasmic reticulum. [EXS. 1996]
Review Protein disulfide isomerase: a multifunctional protein of the endoplasmic reticulum.
Luz JM, Lennarz WJ. EXS. 1996; 77:97-117.
Review Enzymatic catalysis of disulfide formation. [Protein Expr Purif. 1994]
Review Enzymatic catalysis of disulfide formation.
Noiva R. Protein Expr Purif. 1994 Feb; 5(1):1-13.

See reviews... See all...

Cited by 13 PubMed Central articles

Calreticulin-dependent recycling in the early secretory pathway mediates optimal peptide loading of MHC class I molecules. [EMBO J. 2009]
Calreticulin-dependent recycling in the early secretory pathway mediates optimal peptide loading of MHC class I molecules.
Howe C, Garstka M, Al-Balushi M, Ghanem E, Antoniou AN, Fritzsche S, Jankevicius G, Kontouli N, Schneeweiss C, Williams A, et al. EMBO J. 2009 Dec 2; 28(23):3730-44. Epub 2009 Oct 22.
The subcellular distribution of multigene family 110 proteins of African swine fever virus is determined by differences in C-terminal KDEL endoplasmic reticulum retention motifs. [J Virol. 2004]
The subcellular distribution of multigene family 110 proteins of African swine fever virus is determined by differences in C-terminal KDEL endoplasmic reticulum retention motifs.
Netherton C, Rouiller I, Wileman T. J Virol. 2004 Apr; 78(7):3710-21.
The majority of duck hepatitis B virus reverse transcriptase in cells is nonencapsidated and is bound to a cytoplasmic structure. [J Virol. 2000]
The majority of duck hepatitis B virus reverse transcriptase in cells is nonencapsidated and is bound to a cytoplasmic structure.
Yao E, Gong Y, Chen N, Tavis JE. J Virol. 2000 Sep; 74(18):8648-57.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Analysis of the retention signals of two resident luminal endoplasmic reticulum ...
Analysis of the retention signals of two resident luminal endoplasmic reticulum proteins by in vitro mutagenesis.
J Biol Chem. 1991 Apr 5 ;266(10):6015-8.

PubMed
Slowly progressive insulin-dependent diabetes mellitus associated with perniciou...
Slowly progressive insulin-dependent diabetes mellitus associated with pernicious anemia.
Intern Med. 2010 ;49(2):155-9. Epub 2010 Jan 15 .

PubMed
Vitamin D levels, lung function, and steroid response in adult asthma.
Vitamin D levels, lung function, and steroid response in adult asthma.
Am J Respir Crit Care Med. 2010 Apr 1 ;181(7):699-704. Epub 2010 Jan 14 .

PubMed
The effect of cuts in reimbursement on stroke outcome: a nationwide population-b...
The effect of cuts in reimbursement on stroke outcome: a nationwide population-based study during the period 1998 to 2007.
Stroke. 2010 Mar ;41(3):504-9. Epub 2010 Jan 14 .

PubMed
Comparative immunohistochemical study of four monoclonal antibodies directed aga...
Comparative immunohistochemical study of four monoclonal antibodies directed against ovarian carcinoma-associated antigens.
Int J Gynecol Pathol. 1991 ;10(1):15-25.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: