Cryobiology. 2010 Jun;60(3):245-61. doi: 10.1016/j.cryobiol.2010.01.001. Epub 2010 Jan 11.

Comparative analysis of transcriptional responses to the cryoprotectants, dimethyl sulfoxide and trehalose, which confer tolerance to freeze-thaw stress in Saccharomyces cerevisiae.

Momose Y, Matsumoto R, Maruyama A, Yamaoka M.

Source

International Patent Organism Depositary, National Institute of Advanced Industrial Science and Technology, Ibaraki 305-8566, Japan. y.momose@aist.go.jp

Abstract

We have used microarray analysis to monitor the gene expression profile of Saccharomyces cerevisiae BY4743 in the presence of the cryoprotectants, dimethyl sulfoxide (Me(2)SO) and trehalose. Analysis of these profiles suggests that both cryoprotectants increased the expression of genes involved in protein synthesis, ribosomal biogenesis, fatty acid biosynthesis, ergosterol biosynthesis, cell wall biosynthesis, and cellular accumulation of low molecular compounds such as glycerol, arginine and proline. Cryoprotectant treatment reduced the expression of genes involved in the beta-oxidation of fatty acids. In addition, Me(2)SO increased the expression of genes involved in protein refolding and trehalose increased the expression of genes involved in spore formation. This study supported that exposure to cryoprotectants prior to freezing not only reduce the freeze-thaw damage but also provide various process to the recovery from freeze-thaw damage.

PMID:: 20067782; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

Labome Researcher Resource - ExactAntigen/Labome

Molecular Biology Databases

Supplemental Content

Save items

Related citations in PubMed

Membrane stabilization during freezing: the role of two natural cryoprotectants, trehalose and proline. [Cryobiology. 1985]
Membrane stabilization during freezing: the role of two natural cryoprotectants, trehalose and proline.
Rudolph AS, Crowe JH. Cryobiology. 1985 Aug; 22(4):367-77.
Microarray analyses of the metabolic responses of Saccharomyces cerevisiae to organic solvent dimethyl sulfoxide. [J Ind Microbiol Biotechnol. 2003]
Microarray analyses of the metabolic responses of Saccharomyces cerevisiae to organic solvent dimethyl sulfoxide.
Zhang W, Needham DL, Coffin M, Rooker A, Hurban P, Tanzer MM, Shuster JR. J Ind Microbiol Biotechnol. 2003 Jan; 30(1):57-69. Epub 2003 Jan 3.
Cryoprotectants lead to phenotypic adaptation to freeze-thaw stress in Lactobacillus delbrueckii ssp. bulgaricus CIP 101027T. [Cryobiology. 2000]
Cryoprotectants lead to phenotypic adaptation to freeze-thaw stress in Lactobacillus delbrueckii ssp. bulgaricus CIP 101027T.
Panoff JM, Thammavongs B, Guéguen M. Cryobiology. 2000 May; 40(3):264-9.
Review Stress-tolerance of baker's-yeast (Saccharomyces cerevisiae) cells: stress-protective molecules and genes involved in stress tolerance. [Biotechnol Appl Biochem. 2009]
Review Stress-tolerance of baker's-yeast (Saccharomyces cerevisiae) cells: stress-protective molecules and genes involved in stress tolerance.
Shima J, Takagi H. Biotechnol Appl Biochem. 2009 May 29; 53(Pt 3):155-64. Epub 2009 May 29.
Review Molecular biology of trehalose and the trehalases in the yeast Saccharomyces cerevisiae. [Prog Nucleic Acid Res Mol Biol...]
Review Molecular biology of trehalose and the trehalases in the yeast Saccharomyces cerevisiae.
Nwaka S, Holzer H. Prog Nucleic Acid Res Mol Biol. 1998; 58:197-237.

See reviews... See all...

Cited by 1 PubMed Central article

Enhancement of the proline and nitric oxide synthetic pathway improves fermentation ability under multiple baking-associated stress conditions in industrial baker's yeast. [Microb Cell Fact. 2012]
Enhancement of the proline and nitric oxide synthetic pathway improves fermentation ability under multiple baking-associated stress conditions in industrial baker's yeast.
Sasano Y, Haitani Y, Hashida K, Ohtsu I, Shima J, Takagi H. Microb Cell Fact. 2012 Apr 1; 11:40. Epub 2012 Apr 1.

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
MedGen
Related information in MedGen
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Comparative analysis of transcriptional responses to the cryoprotectants, dimeth...
Comparative analysis of transcriptional responses to the cryoprotectants, dimethyl sulfoxide and trehalose, which confer tolerance to freeze-thaw stress in Saccharomyces cerevisiae.
Cryobiology. 2010 Jun ;60(3):245-61. doi: 10.1016/j.cryobiol.2010.01.001. Epub 2010 Jan 11 .

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: