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J Cell Biol. 2010 Jan 11;188(1):101-14. doi: 10.1083/jcb.200904075.

The conserved oligomeric Golgi complex is involved in double-membrane vesicle formation during autophagy.

Author information

  • 1Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA.

Abstract

Macroautophagy is a catabolic pathway used for the turnover of long-lived proteins and organelles in eukaryotic cells. The morphological hallmark of this process is the formation of double-membrane autophagosomes that sequester cytoplasm. Autophagosome formation is the most complex part of macroautophagy, and it is a dynamic event that likely involves vesicle fusion to expand the initial sequestering membrane, the phagophore; however, essentially nothing is known about this process including the molecular components involved in vesicle tethering and fusion. In this study, we provide evidence that the subunits of the conserved oligomeric Golgi (COG) complex are required for double-membrane cytoplasm to vacuole targeting vesicle and autophagosome formation. COG subunits localized to the phagophore assembly site and interacted with Atg (autophagy related) proteins. In addition, mutations in the COG genes resulted in the mislocalization of Atg8 and Atg9, which are critical components involved in autophagosome formation.

PMID:
20065092
[PubMed - indexed for MEDLINE]
PMCID:
PMC2812853
Free PMC Article

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