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J Am Chem Soc. 2010 Feb 3;132(4):1218-9. doi: 10.1021/ja909298v.

Modulation of buried ionizable groups in proteins with engineered surface charge.

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  • 1Facultad de Ciencias, Departamento de Quimica Fisica, Universidad de Granada, 18071-Granada, Spain.


Recent work has shown that proteins can tolerate hydrophobic-to-ionizable-residue mutations. Here, we provide experimental evidence that the essential properties (pK value, protonation state, local dynamics) of buried ionizable groups in proteins can be efficiently modulated through the rational design of the surface charge distribution, thus paving the way for the protein engineering exploitation of charge burial.

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