A, B. Left/active lever presses (A) and right/inactive lever presses (B) during the 15-second timeout per daily cocaine self-administration session. Irradiated rats pressed significantly press more on the drug-paired lever during the timeout on the first two self-administration sessions, but thereafter did not press significantly more than sham rats (A). Irradiated rats did not significantly press more on the inactive lever than sham rats during any session (B). C, D. Left/active lever presses (C) and right/inactive lever presses (D) during the 15-second timeout per daily sucrose self-administration session. Irradiated and sham rats pressed the same amount on the food-paired lever (C) and the inactive lever (D) during the timeout period. Data presented as mean ± S.E.M. *p<0.05.

A. Irradiated rats did not differ from sham rats in the time it took to obtain 100 sucrose pellets during acquisition of an FR1 schedule during food training. B. Basal locomotion was not different between irradiated and sham rats. C. Irradiated rats were not different from sham rats in sucrose pellet self-administration on a FR1, FR3, or FR5 schedule. D, E. Sucrose pellet self-administration on a progressive-ratio was not different between irradiated and sham rats when rats were food restricted during testing days (D) or when not food restricted (E). F. Irradiated rats did not differ from sham rats in their ability to extinguish responding on the formerly sucrose-paired lever. G. Irradiated rats did not differ from sham rats in responding to cues formerly paired with sucrose administration. H. Irradiated rats did not differ from sham rats in responding to presentation of sucrose pellets. Data presented as mean ± S.E.M.

A. Irradiated rats did not differ from sham rats in the time it took to obtain 100 sucrose pellets during acquisition of an FR1 schedule during food training. B. Cranial irradiation 4 weeks prior increased the amount of cocaine self-administered at a 0.5 mg/kg infusion dose under both FR1 and FR5 schedules. C. Irradiated rats self-administered more cocaine, causing a vertical shift in the dose-response curve. D. Dose-response data converted into dose-intake curves by multiplying infusions by dose show irradiated rats took more significantly more cocaine. E. Irradiated rats worked harder to get their last infusion of cocaine, suggesting they find cocaine more rewarding. Data presented as mean ± S.E.M.*p<0.05, **p<0.01, ***p<0.001.

A. Context-induced reinstatement. Irradiated rats did not press more than sham rats on the formerly drug-paired lever when re-exposed to the self-administration chamber after 4 weeks of abstinence. B. Cue-induced reinstatement. Irradiated rats did not differ from sham rats in drug-seeking in response to drug cues. C. Drug-induced reinstatement. Response to pressing the left, formerly drug-paired lever (left-most pair of bars) or right, formerly drug-unpaired lever (right-most pair of bars) after i.p. injection of saline or 5 or 15 mg/kg cocaine in sham and irradiated rats. Irradiated rats did not differ from sham rats in drug-seeking in response to low dose cocaine injections. Right lever presses are presented but are under five for both irradiated and sham groups at each dose of cocaine. D. Footshock stress-induced reinstatement. Lever pressing between irradiated and sham-irradiated rats in response to footshock stress was not significantly different. E. Locomotion assessment. Irradiated rats were not impaired in either basal locomotion or cocaine-induced locomotion (15 mg/kg i.p.). Data presented as mean ± S.E.M.

A. Self-administration of cocaine. Cocaine self-administration in each group prior to irradiation. Rat were then divided to make two statistically similar groups and assigned to CSA-WD/IRR or CSA-WD/Sham groups. B, C. Context-induced reinstatement. Irradiation after CSA lead to enhanced context-dependent reinstatement. Irradiated rats pressed more on the formerly drug-paired lever (B) and took more sessions to extinguish lever pressing on the formerly drug-paired lever (C) when re-exposed daily to the self-administration chamber in the absence of reinforcement after 4 weeks of abstinence. D. Cue-induced reinstatement. Irradiated rats had non-statistical trend towards pressing both the formerly drug-paired lever and the inactive lever more than sham-irradiated rats in response to drug-cues. E. Drug-induced reinstatement. Response to pressing the left, formerly drug-paired lever (left-most pair of bars) or right, formerly drug-unpaired lever (right-most pair of bars) after i.p. injection of saline or 5 or 15 mg/kg cocaine in sham and irradiated rats. Irradiated rats had nonstatistical trend towards pressing the formerly drug-paired left lever more in response to low dose (5 mg/kg i.p.) cocaine exposure. Right lever presses are presented but are under five for both irradiated and sham groups at every dose of cocaine. F. Footshock stress-induced reinstatement. Lever pressing between irradiated and sham-irradiated rats in response to footshock stress. G. Locomotion assessment. Irradiated rats were not impaired in either basal locomotion or cocaine-induced locomotion (15 mg/kg i.p.). Data presented as mean ± S.E.M. *p<0.05, **p<0.01.

A. Representative images at 200× magnification of doublecortin+ (DCX+) immature neurons in the dorsal/anterior SGZ near the apex of the ventral and dorsal dentate gyrus limbs in Sham-CSA and IRR-CSA rats 13 weeks after irradiation. DCX+ staining was almost completely absent in dorsal dentate gyrus sections in irradiated rats. Scale bar = 50 μm. B. Quantitative analysis of DCX+ SGZ cell number at various times after cranial irradiation: cocaine self-administration (CSA) 13 weeks; CSA/progressive-ratio (PR) 9 weeks; sucrose self-administration (SSA) 10 weeks; CSA-withdrawal (WD) 5 weeks. Time line of experiments is provided in Figure 1. Regardless of time after irradiation, the number of DCX+ SGZ cells was significantly reduced relative to control rats, with almost complete ablation of DCX+ cells in the dorsal dentate gyrus (Panel A). C, D. Quantitative analysis of DCX+ cell number in relation to distance from Bregma in Sham-CSA and IRR-CSA rats (Panel C) and CSA-WD/Sham and CSA-WD/IRR rats (Panel D). While DCX+ staining was almost completely absent in dorsal dentate gyrus sections (Panel A), this analysis via distance from Bregma reveals that DCX+ staining was more evident in ventral dentate gyrus sections. This likely represents the loss of radiation energy as it passed from the dorsal to ventral regions of the brain. E. Representative photomicrographs from ED1/CD-68 stained sections taken from sham and irradiated rats 3 and 4 weeks after irradiation. Arrows indicate ED1/CD-68+ cells in dentate gyrus; inset shows higher magnification of ED1/CD-68+ cell to highlight one of the characteristic ramified shape presented by activated microglia. Consistent with previous publications, this paradigm of irradiation resulted in modest microglial activation at 3 weeks. This activation was transient as only rare positive ED1/CD-68+ cells were observed at 4 weeks after irradiation and none at later time points (data not shown). Scale bar = 20μm F. Scatterplot of brain levels of cocaine in rats injected with 20 mg/kg cocaine intraperitoneally. There is no significant difference between the means, and no data points were statistically valid to remove as outliers based on the Grubb’s test (GraphPad Prism). Data for B presented as mean ± S.E.M. *p<0.5, **p<0.01, ***p<0.001.

A. IRR-CSA (n=21) and Sham-CSA (n=18) rats were habituated to the animal facility for a week, anesthetized (50 mg/kg sodium pentobarbital, i.p.), irradiated, and left in home cage to recover for 4 weeks (with handling every 3 days). Rats completed food training, were implanted with an intravenous (i.v.) catheter, and allowed to recover for several days. Three weeks of cocaine self-administration (CSA) followed. Rats were then split into groups for progressive-ratio testing (PR) or dose response and reinstatement. PR rats had 6 days of testing and then were sacrificed the next day (IRR n=7, Sham n=5). The remaining rats were given a dose-response test on the 16th day of CSA (IRR n=14, Sham n=13), and then were placed in the home cage for 4 weeks of withdrawal (WD). Daily reinstatement testing followed, which consisted of an extinction session for at least one hour followed by reinstatement testing to several variables as described in methods: the context itself, the cue light, saline, i.p. cocaine (5 or 15 mg/kg), and finally footshock stress. A locomotor test (basal and after a 15 mg/kg cocaine injection) was done on the last day. Rats were sacrificed 24 hrs later. B. The experimental design for IRR-SSA (n=21) and Sham-SSA (n=18) rats was similar to Figure 1A, except that rats were not implanted with an i.v. catheter after food training, and locomotor testing occurred after the last day of food training. Rats completed 3 weeks of sucrose pellet self-administration (SSA), followed by PR for sucrose pellets while food restricted for 3 day and while fed ad libitum for 3 days. Rats were then food restricted and restabilized on FR5 timeout 15 sec sucrose self-administration for 3 days. Reinstatement testing consisted of extinction of sucrose-seeking, cue-induced and sucrose-induced reinstatement testing. C. CSA-WD/IRR (n=15) and CSA-WD/Sham (n=9) rats were irradiated after 3 weeks of CSA, and after 4 weeks of WD coincident with the recovery period, were then tested for reinstatement. All rats were age-matched to the beginning of CSA or SSA. Scale bar = 50 μm. Lightening bolt = 2 days of 10 Gy cranial irradiation, in a 1 cm diameter circle on the dorsal surface of the head to target the hippocampus as previously described (e.g.Snyder et al., 2005). CSA = cocaine self-administration, H = habituation, IRR = irradiation, FT/S = food training and i.v. catheter surgery, R = recovery, sac = sacrifice by intracardial perfusion, SSA = sucrose self-administration, Reinstate = reinstatement testing, PR = progressive-ratio, WD = withdrawal period.