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Biosens Bioelectron. 2010 Mar 15;25(7):1635-9. doi: 10.1016/j.bios.2009.11.028. Epub 2009 Dec 5.

Sensitive giant magnetoresistive-based immunoassay for multiplex mycotoxin detection.

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  • 1Stanford Genome Technology Center, Stanford University, 855 California Ave, Palo Alto, CA 94304, USA.

Abstract

Rapid and multiplexed measurement is vital in the detection of food-borne pathogens. While highly specific and sensitive, traditional immunochemical assays such as enzyme-linked immunosorbent assays (ELISAs) often require expensive read-out equipment (e.g. fluorescent labels) and lack the capability of multiplex detection. By combining the superior specificity of immunoassays with the sensitivity and simplicity of magnetic detection, we have developed a novel multiplex magnetic nanotag-based detection platform for mycotoxins that functions on a sub-picomolar concentration level. Unlike fluorescent labels, magnetic nanotags (MNTs) can be detected with inexpensive giant magnetoresistive (GMR) sensors such as spin-valve sensors. In the system presented here, each spin-valve sensor has an active area of 90 microm x 90 microm, arranged in an 8 x 8 array. Sample is added to the antibody-immobilized sensor array prior to the addition of the biotinylated detection antibody. The sensor response is recorded in real time upon the addition of streptavidin-linked MNTs on the chip. Here we demonstrate the simultaneous detection of multiple mycotoxins (aflatoxins B(1), zearalenone and HT-2) and show that a detection limit of 50 pg/mL can be achieved.

(c) 2009. Published by Elsevier B.V. All rights reserved.

PMID:
20047828
[PubMed - indexed for MEDLINE]
PMCID:
PMC2947153
Free PMC Article
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