[Screening of proteins binding to FXR1P using yeast two-hybrid technique]

Nan Fang Yi Ke Da Xue Xue Bao. 2009 Dec;29(12):2394-6, 2400.
[Article in Chinese]

Abstract

Objective: To screen the proteins interacting with FXR1P for functional investigation of FXR1P.

Methods: The yeast strain AH109 transformed with the recombinant expression vector pGBKT7/FXR1 was mated with the yeast strain Y187 pretransformed with human fetal brain cDNA library. The positive clones were screened and identified by sequence analysis.

Results: The recombinant expression vector pGBKT7/FXR1 was constructed successfully. Five proteins binding to FXR1P were screened from human fetal brain cDNA library using the yeast two-hybrid system, including CMAS, FTH1, GOLGA4, HSD17B1 and CSH1.

Conclusions: These results provide new clues for investigating the biological functions of FXR1P and the pathogenesis of Fragile X syndrome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Autoantigens / genetics
  • Autoantigens / metabolism
  • Estradiol Dehydrogenases / genetics
  • Estradiol Dehydrogenases / metabolism
  • Ferritins / genetics
  • Ferritins / metabolism
  • Gene Library
  • Humans
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism
  • Oxidoreductases
  • Protein Binding*
  • Protein Interaction Domains and Motifs / genetics*
  • RNA-Binding Proteins / genetics*
  • RNA-Binding Proteins / metabolism
  • Two-Hybrid System Techniques*

Substances

  • Autoantigens
  • FXR1 protein, human
  • GOLGA4 protein, human
  • Membrane Proteins
  • RNA-Binding Proteins
  • Ferritins
  • FTH1 protein, human
  • Oxidoreductases
  • Estradiol Dehydrogenases
  • HSD17B1 protein, human