Summary of functionally important regions of conserved yeast TFIIF subunits. (A, B) The S. mikatae Tfg1 and S. cerevisiae Tfg2 subunits. Shown are the regions homologous to the human Rap74 and Rap30 dimerization domain, the yeast-specific dimerization domain insertions and the winged helix domains. Colours summarize the functionally important regions from Supplementary Table S1. Black, essential for yeast growth; orange, temperature/cold sensitive or slow growth when deleted; white, not required for normal growth. The numbers indicate the amino acid residues in which FeBABE was linked and ^* indicates the positions of FeBABE attachments that generate cleavage of Rpb1 and/or Rpb2 in PICs.

Model of the yeast TFIIF dimerization domain binding to Pol II in the PIC. (A) FeBABE cleavage data for all TFIIF-FeBABE derivatives linked to the TFIIF dimerization domain. The cleavage data are from Supplementary Table S2. Ten residues on either side of the calculated cleavage site in Rpb1 and Rpb2 are coloured blue and Pol II is shown in the PIC model (Kostrewa et al, 2009). TBP, green; TFIIB, yellow. (B) Model for the TFIIF dimerization domain binding to the Pol II lobe domain. On the basis of the cleavage data from individual TFIIF-FeBABE derivatives and manual docking, the indicated position of TFIIF can account for the observed Pol II cleavage patterns. Orange, Tfg1; Red, Tfg2.

Mapping the location of the Tfg1 and Tfg2 linkers on Pol II in the PIC. (A) FeBABE cleavage data from Supplementary Table S2A showing the positions of Rpb2 cleavage from FeBABE positioned at Tfg1 residues N480 and A541. Cleavage sites are highlighted in purple. (B) FeBABE cleavage data from Supplementary Table S2B showing the positions of Rpb2 cleavage from FeBABE positioned at Tfg2 Q239 (blue) and N274, A285 (violet). (C, D) PIC model with the combined deduced positions of the TFIIF dimerization domain, the Tfg2 winged helix domain, and the Tfg2 flexible linker represented as a dotted line.

Mutations in the TFIIF dimerization and winged helix domains lead to changes in transcription start site. (A) The Tfg2 winged helix contributes to Pol II–TFIIF binding. Pol II of 1 μg attached to anti-Flag beads was mixed with the indicated amounts of purified TFIIF and Pol II-associated TFIIF recovered by IP was assayed by western blot. Variable amounts of purified TFIIF in lanes 1–5 were used to quantitate TFIIF-associated Pol II in lanes 7–10. The input levels of wild type (WT) and mutant TFIIF derivatives gave equivalent signals in a western blot assay (not shown). (B) Mutation of the Tfg2 winged helix alters start site usage. Nuclear extract depleted for TFIIF was supplemented with purified TFIIF and transcription at the HIS4 promoter was visualized by primer extension. (C) Mutations in the TFIIF dimerization domain, which alter the transcription start site. The TFIIF dimerization domain-PIC model showing residues in green that when mutated shift the transcription start site upstream from the normal position (Ghazy et al, 2004; Freire-Picos et al, 2005). The Rpb9 subunit is shown in yellow and the two bound Zn atoms in Brown. (D) Primer extension analysis of in vivo ADH1 mRNAs from selected Tfg1 and Tfg2 mutations.

TFIIF-FeBABE-dependent cleavage of Rpb2 in the PIC. PICs were formed at the HIS4 promoter using nuclear extract supplemented with the indicated TFIIF-FeBABE derivatives, hydroxyl radical cleavage activated with H₂O₂, and the resulting cleavage products visualized by western blot. ^*Indicates reproducibly observed cleavage products compared with a reaction using a non-cysteine-containing TFIIF. (A) Cleavage from FeBABE inserted in the TFIIF dimerization domain and Tfg1 linker regions. Left panel uses N-terminal Flag-tagged Rpb2. All other assays use C-terminal Flag-tagged Rpb2. (B, C) Cleavage from FeBABE inserted in the Tfg2 linker and winged helix regions.

Position of the Tfg2 winged helix domain in the PIC. (A) Combined cleavage data for all TFIIF-FeBABE derivatives linked to the Tfg2 winged helix domain. Ten residues on either side of the calculated cleavage sites in Rpb2 are coloured blue. One arrow points to α-helices 2 and 3 in the protrusion domain (Cramer et al, 2001), which cannot be assayed for cleavage because they are at the very N-terminus of Rpb2. The base pair highlighted in red and blue indicates the most upstream site of DNA melting in the human system (Miller and Hahn, 2006; Kostrewa et al, 2009). (B) Model for the position of the winged helix domain that best fits the cleavage data. α-helix 3 of the WH domain (the DNA recognition helix) is indicated.

Comparison of TFIIF binding to purified Pol II and to Pol II in PICs. TFIIF-FeBABE derivatives were mixed with purified Pol II containing a C-terminal Flag-tagged Rpb2 subunit, and hydroxyl radical production was activated and cleavage visualized by western blot. This was done in parallel with the same TFIIF derivatives incorporated in PICs. (A) FeBABE linked to the TFIIF dimerization domain. (B) FeBABE linked to the Tfg2 winged helix domain. (C) The Pol II–TFIIF dimerization domain model from Figure 3 with cleavage observed in reactions containing purified Pol II and the Tfg2 winged helix-FeBABE derivatives. The cleaved region, highlighted in blue, shows that the winged helix binds near the Pol II lobe in the Pol II–TFIIF complex. Cleavage data are from Supplementary Table S3.