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Ecotoxicol Environ Saf. 2010 Mar;73(3):262-9. doi: 10.1016/j.ecoenv.2009.12.001. Epub 2009 Dec 23.

The use of marine benthic diatoms in a growth inhibition test with spiked whole-sediment.

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  • 1Andalusia Institute of Marine Science (CSIC), Campus Rio San Pedro, Puerto Real (Cádiz), Spain. aourell.mauffret@icman.csic.es

Abstract

The benthic diatom Cylindrotheca closterium was exposed to whole-sediment spiked with the synthetic surfactant Linear Alkylbenzene Sulphonate (LAS), as commercial mixture or individual homologues (C10-LAS, C11-LAS or C12-LAS). Separately, the diatoms were exposed to C12-LAS in a water-only system. The algal growth was determined after 72 h. The 72-h ErC50 values ranged from <or=4 to 29 mg/kg sediment, suggesting that C. closterium was sensitive to LAS. The outcomes were reproducible with a variation factor lower than 3, showing the reproducibility of the method. The test set up was cost effective, used little bench space and employed a micro-phytobenthos species that fulfilled most of the criteria for a suitable test species in sediment systems. The 72-h ErC50 obtained in a water-only system (0.141 mg/L) was ca. 2 orders of magnitude lower (0.002 mg/L). This was likely due to an indirect effect of the use of fresh sediment.

(c) 2009 Elsevier Inc. All rights reserved.

PMID:
20031213
[PubMed - indexed for MEDLINE]
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