Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
BMC Biotechnol. 2009 Dec 21;9:102. doi: 10.1186/1472-6750-9-102.

RNA degradation compromises the reliability of microRNA expression profiling.

Author information

  • 1Genomics Core Facility, EMBL, Meyerhofstrasse 1 D-69117 Heidelberg, Germany. ibberson@embl.de

Abstract

BACKGROUND:

MicroRNAs are small non-coding RNAs that post-transcriptionally regulate gene expression and their expression is frequently altered in human diseases, including cancer. To correlate clinically relevant parameters with microRNA expression, total RNA is frequently prepared from samples that were archived for various time periods in frozen tissue banks but, unfortunately, RNA integrity is not always preserved in these frozen tissues. Here, we investigate whether experimentally induced RNA degradation affects microRNA expression profiles.

RESULTS:

Tissue samples were maintained on ice for defined time periods prior to total RNA extraction, which resulted in different degrees of RNA degradation. MicroRNA expression was then analyzed by microarray analysis (miCHIP) or microRNA-specific real-time quantitative PCR (miQPCR). Our results demonstrate that the loss of RNA integrity leads to in unpredictability of microRNA expression profiles for both, array-based and miQPCR assays.

CONCLUSION:

MicroRNA expression cannot be reliably profiled in degraded total RNA. For the profiling of microRNAs we recommend use of RNA samples with a RNA integrity number equal to or above seven.

PMID:
20025722
[PubMed - indexed for MEDLINE]
PMCID:
PMC2805631
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for BioMed Central Icon for PubMed Central
    Loading ...
    Write to the Help Desk