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Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400044, People's Republic of China.
Most common method for isolation of light-harvesting 2 complex (LH2) is very time-consuming. In our present work functional LH2 complex was one-step rapidly purified from Rhodobacter sphaeroides by metal-ion affinity chromatography. The purified LH2 complex exhibited high activity in light absorption and energy transfer. Our present work provides an efficient approach for preparation of functional LH2 complex.
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