Display Settings:

Format

Send to:

Choose Destination
Stem Cells. 2010 Mar 31;28(3):489-500. doi: 10.1002/stem.279.

Maximizing functional photoreceptor differentiation from adult human retinal stem cells.

Author information

  • 1Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada. tinoue@ophthal.med.osaka-u.ac.jp

Abstract

Retinal stem cells (RSCs) are present in the ciliary margin of the adult human eye and can give rise to all retinal cell types. Here we show that modulation of retinal transcription factor gene expression in human RSCs greatly enriches photoreceptor progeny, and that strong enrichment was obtained with the combined transduction of OTX2 and CRX together with the modulation of CHX10. When these genetically modified human RSC progeny are transplanted into mouse eyes, their retinal integration and differentiation is superior to unmodified RSC progeny. Moreover, electrophysiologic and behavioral tests show that these transplanted cells promote functional recovery in transducin mutant mice. This study suggests that gene modulation in human RSCs may provide a source of photoreceptor cells for the treatment of photoreceptor disease.

PMID:
20014120
[PubMed - indexed for MEDLINE]
PMCID:
PMC2933833
Free PMC Article

Images from this publication.See all images (5)Free text

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for John Wiley & Sons, Inc. Icon for PubMed Central
    Loading ...
    Write to the Help Desk