Format

Send to:

Choose Destination
See comment in PubMed Commons below
Methods Mol Biol. 2010;604:55-71. doi: 10.1007/978-1-60761-444-9_5.

Target-decoy search strategy for mass spectrometry-based proteomics.

Author information

  • 1Department of Cell Biology, Harvard Medical School, Boston, MA, USA.

Abstract

Accurate and precise methods for estimating incorrect peptide and protein identifications are crucial for effective large-scale proteome analyses by tandem mass spectrometry. The target-decoy search strategy has emerged as a simple, effective tool for generating such estimations. This strategy is based on the premise that obvious, necessarily incorrect "decoy" sequences added to the search space will correspond with incorrect search results that might otherwise be deemed to be correct. With this knowledge, it is possible not only to estimate how many incorrect results are in a final data set but also to use decoy hits to guide the design of filtering criteria that sensitively partition a data set into correct and incorrect identifications.

PMID:
20013364
[PubMed - indexed for MEDLINE]
PMCID:
PMC2922680
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Springer Icon for PubMed Central
    Loading ...
    Write to the Help Desk