The plus strand is discontinuous in a subpopulation of unintegrated HIV-1 DNA

Arch Virol. 1991;116(1-4):133-41. doi: 10.1007/BF01319237.

Abstract

During reverse transcription the synthesis of plus strand viral DNA is initiated from an RNA polypurine primer immediately upstream of the U 3 region. The polypurine tract (PPT) sequence at this site is in HIV-1 also present in the middle of the genome. Here we demonstrate that a subpopulation of linear unintegrated HIV-1 DNA has a discontinuity in the plus strand within less than 50 bp from this central PPT, consistent with its utilization as a plus strand initiation site.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Replication / genetics*
  • DNA, Viral / genetics*
  • Electrophoresis, Polyacrylamide Gel
  • HIV-1 / genetics*
  • HIV-1 / growth & development
  • Immunoblotting
  • Nucleic Acid Hybridization

Substances

  • DNA, Viral