Arch Virol. 1991;116(1-4):133-41.

The plus strand is discontinuous in a subpopulation of unintegrated HIV-1 DNA.

Source

Department of Virology, National Institute of Public Health, Oslo, Norway.

Abstract

During reverse transcription the synthesis of plus strand viral DNA is initiated from an RNA polypurine primer immediately upstream of the U 3 region. The polypurine tract (PPT) sequence at this site is in HIV-1 also present in the middle of the genome. Here we demonstrate that a subpopulation of linear unintegrated HIV-1 DNA has a discontinuity in the plus strand within less than 50 bp from this central PPT, consistent with its utilization as a plus strand initiation site.

PMID:: 2001174; [PubMed - indexed for MEDLINE]

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Research Support, Non-U.S. Gov't

MeSH Terms

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DNA, Viral

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