Human papillomavirus 5 and 8 E6 downregulate interleukin-8 secretion in primary human keratinocytes

J Gen Virol. 2010 Apr;91(Pt 4):888-92. doi: 10.1099/vir.0.016527-0. Epub 2009 Dec 9.

Abstract

Human papillomaviruses (HPVs) of the genus Betapapillomavirus appear to be involved in the early stages of skin cancer development, since both the prevalence and viral load are higher in precancerous actinic keratoses than in skin cancers. Interleukin-8 (IL-8) is an inflammatory cytokine that serves to alert the surrounding tissue after UV-induced damage. We examined the effects of the E2, E6 and E7 proteins of HPV8 and the E6 proteins of various HPV genotypes on IL-8 secretion from primary keratinocytes. HPV5 and HPV8 E6 showed the highest downregulation of basal IL-8 secretion. HPV8 E6 also negatively modulated IL-8 mRNA expression and protein secretion upon UVB irradiation. The downregulation of IL-8 in actinic keratoses may weaken the response to UV-induced damage and thus favour the accumulation of UVB-induced mutations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Down-Regulation
  • Humans
  • Interleukin-8 / antagonists & inhibitors*
  • Interleukin-8 / genetics
  • Interleukin-8 / metabolism
  • Keratinocytes / metabolism*
  • Keratinocytes / radiation effects
  • Keratosis, Actinic / etiology
  • Oncogene Proteins, Viral / physiology*
  • RNA, Messenger / analysis
  • Skin Neoplasms / etiology
  • Ultraviolet Rays

Substances

  • E6 protein, Human papillomavirus type 8
  • E6 protein, human papillomavirus type 5
  • Interleukin-8
  • Oncogene Proteins, Viral
  • RNA, Messenger