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Mol Cell. 2009 Dec 11;36(5):768-81. doi: 10.1016/j.molcel.2009.11.011.

Rrp17p is a eukaryotic exonuclease required for 5' end processing of Pre-60S ribosomal RNA.

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  • 1Rockefeller University, New York, NY 10065, USA.

Abstract

Ribosomal processing requires a series of endo- and exonucleolytic steps for the production of mature ribosomes, of which most have been described. To ensure ribosome synthesis, 3' end formation of rRNA uses multiple nucleases acting in parallel; however, a similar parallel mechanism had not been described for 5' end maturation. Here, we identify Rrp17p as a previously unidentified 5'-3' exonuclease essential for ribosome biogenesis, functioning with Rat1p in a parallel processing pathway analogous to that of 3' end formation. Rrp17p is required for efficient exonuclease digestion of the mature 5' ends of 5.8S(S) and 25S rRNAs, contains a catalytic domain close to its N terminus, and is highly conserved among higher eukaryotes, being a member of a family of exonucleases. We show that Rrp17p binds late pre-60S ribosomes, accompanying them from the nucleolus to the nuclear periphery, and provide evidence for physical and functional links between late 60S subunit processing and export.

PMID:
20005841
[PubMed - indexed for MEDLINE]
PMCID:
PMC2806520
Free PMC Article
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