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J Appl Microbiol. 2010 Jul;109(1):116-27. doi: 10.1111/j.1365-2672.2009.04633.x. Epub 2009 Nov 23.

Physiological and molecular analysis of the stress response of Saccharomyces cerevisiae imposed by strong inorganic acid with implication to industrial fermentations.

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  • 1Department of Genetics, Federal University of Pernambuco, Recife, Pernambuco, Brazil.

Abstract

AIMS:

This work aimed to identify the molecular mechanism that allows yeast cells to survive at low pH environments such as those of bioethanol fermentation.

METHODS AND RESULTS:

The industrial strain JP1 cells grown at pH 2 was evaluated by microarray analysis showing that most of the genes induced at low pH were part of the general stress response (GSR). Further, an acid-tolerant yeast mutant was isolated by adaptive selection that was prone to grow at low pH in inorganic but weak organic acid. It showed higher viability under acid-temperature synergistic treatment. However, it was deficient in some physiological aspects that are associated with defects in protein kinase A (PKA) pathway. Microarray analysis showed the induction of genes involved in inhibition of RNA and protein synthesis.

CONCLUSIONS:

The results point out that low pH activates GSR, mainly heat shock response, that is important for long-term cell survival and suggest that a fine regulatory PKA-dependent mechanism that might affect cell cycle in order to acquire tolerance to acid environment.

SIGNIFICANCE AND IMPACT OF THE STUDY:

These findings might guide the construction of a high-fermentative stress-tolerant industrial yeast strain that can be used in complex industrial fermentation processes.

© 2009 The Authors. Journal compilation © 2009 The Society for Applied Microbiology.

[PubMed - indexed for MEDLINE]
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