Representative photomicrographs of H.E-stained coronal brain sections from infected animals at 2, 5, 7, 14, 30 and 60 d p.i. Specific areas of the brain are shown, including the first cranial (olfactory) nerve/olfactory bulb ((ON)/OB; a1–f1), sensory nucleus of the trigeminal nerve (sn5; b2–f2), spinal root of the trigeminal nerve (sp5, a3–f3) and the solitary and vagus nuclei (SolN/XN; a1–a4; Bar= 50 µm). The progression of inflammation in the various brains stem regions and their associated nerves demonstrating a predominant neutrophil infiltrate up to 7 d p.i, which changed to a lymphocytic and plasmacytic infiltrate at 14 d p.i.

During the acute infection (7 d p.i), inflammatory infiltration within the solitary nuclei (SolN) and vagus nucleus (XN) were composed of mostly neutrophils, and demonstrated abundant nuclear debris, cell lysis and neurons with intranuclear viral inclusion body. Coronal section at 7.20 mm caudal to the bregma stained with (a) H.E or immunostained for (b) myeloperoxidase, (c) Mac-2, and (d) Caspase 3 demonstrated the presence of neutrophils, activated macrophages and apoptotic leukocytes in this region (Bar= 50 µm).

Photomicrographs of cortical brain sections, 1.82 mm caudal to the Bregma, obtained from infected animals at various d p.i demonstrating the progressive astroglial activation in the cerebral cortex with no parenchymal loss and scar formation in the brainstem consequent to HSV-1 infection. Low magnification sections immunostained for GFAP expression are presented from (a1) uninfected animals and (a2–a3) corresponding high magnification micrographs denoting normal structure and numbers of GFAP positive cells in the uninfected brain. Similar low magnification coronal sections taken at (b1) 14 d p.i, and (c1) 30 d p.i showed an chronic increase in GFAP signal across many brain structures (Bar= 2mm). (b2–b3) At 1 4 d p.i, magnification of the parietal cortex (b2) and amygdaloid nuclei (b3) showed a locally extensive astrocyte reaction (Bar = 50 µm), particularly in the amygdaloid nuclei astrogliosis was located around areas of lymphocytic perivasculitis (arrow). (c2–c3) Astrogliosis at 30 d p.i (Bar= 2mm) was observed as hypertrophic astrocytes (c2) possessing prominent cell processes and strong GFAP-expression indicative of glia scar formation (c3, Bar = 50 µm). (d–e) Similar astrogliosis was observed in the brain stem regions. Brain cross sections at 7.76 mm caudal to the Bregma from uninfected animals (d) compared to infected brains at 30 d p.i (e) showed a diffuse astrogliosis in the infected brain stem. Severe astrogliosis was observed in the caudal part of the spinal trigeminal nucleus and glia scar formation (arrow) in the spinal trigeminal track (Bar= 0.5 mm).

Low magnification photomicrographs presenting the brain of a mouse showing severe atrophy and cavitations of cortical structures at 60 d p.i. (a1) H.E. stained coronal section, 1.82 mm caudal to Bregma, from infected and (a2) uninfected age-matched animal demonstrating bilateral chronic temporal cortical atrophy with cavitations (arrows). (b1) Adjacent sections (−1.82 mm) stained with GFAP shown a diffuse severe astrogliosis and hypertrophic astrocytes. Notice the intense and extensive GFAP-expression demonstrating hypertrophic astrocytes in a glial scar formation, (b2) compared to the same region in an uninfected animals. (c1) Similar H.E. stained coronal sections at 3.16 mm caudal to bregma show cavitations and atrophy of the entorhinal cortex (arrows) compared to (c2) uninfected age-matched controls. (d1) adjacent sections stained with GFAP showed a diffuse severe astrogliosis compared (d2) to similar region in uninfected brains (Bar= 2.0 mm). (e1) High power examination of the H.E stained coronal brain section, presented in a1, exhibited severe loss of neurons of the CA1, CA2, and CA3 regions of hippocampus compared to (e2) age-matched, uninfected control controls (Bar= 0.5 mm). Py = pyramidal neurons, GrDG = granular cell layer of the dentate gyrus. (f1) This loss of neuronal structure in the hippocampus was associated with severe astrogliosis, characterized by a strong expression of GFAP in concurring CA areas compared to (f2) similar regions in uninfected, age-matched control brains. (Bar = 0.5 mm).

Photomicrographs of representative H.E-stained coronal brain sections at 1.82 mm caudal to the Bregma. a1, b1, and c1 are shown as low magnification at 7, 30, and 60 d p.i, respectively, from which descriptive lesions are presented in the subsequent figures (Bar = 2mm). (a2–a4) During the initial stages of acute infection (7 d p.i), signs of severe degeneration and ultimately necrosis of infected neurons were observed (a2; arrow). Scattered CD3 (a3) and Mac-2 (a4) positive cells were present in the leptomeninges (arrows; Bar = 50µm). (b2–b4) During the chronic inflammatory phase, 30 d p.i, the piriform cortex exhibited severe atrophy with a marked loss of neurons and neuropile that is replaced by inflammatory infiltrate (b2). The infiltrate was composed of moderate numbers of CD-3 (b3) and Mac-2 (b4) expressing cells (lymphocytes and macrophages, respectively; Bar=50µm). (c2–c4) At 60 d p.i, the piriform cortex and amygdaloid nuclei showed extensive loss of neural tissue with cavitations (c2). The inflammatory infiltrates that demarcated the lesion (c2) were composed of large numbers of CD-3 (c3) and Mac-2 (c4) expressing cells (lymphocytes and macrophages, respectively; Bar=50µm).

Changes in the brain stem at 14 d p.i are shown in a representative coronal section at 5.52 mm caudal to the Bregma, demonstrating a severe sub-chronic inflammatory response in the spinal trigeminal tract/ facial nerve root. (a) An H.E.-stained preparation demonstrating the presence of moderate numbers of plasma cells (arrow head) and Russell bodies (arrow), (b) moderate numbers CD-3 lymphocytes, and (c) large numbers of Mac-2 positive phagocytes (Bar= 50 µm).

Single cell suspensions of brain tissue obtained from HSV-1-infected mice (5 animals, 30 d p.i) were layered on a 70% Percoll cushion. (a) Brain leukocytes banded at the 30–70% Percoll interface were collected, labeled with mouse antibodies specific for CD45 and CD11b, and sorted into four populations using FACS: A- CD45⁻CD11b⁻ non-myeloid cells; B- CD45^hiCD11b⁻ predominantly lymphocytes; C- CD45^hiCD11b⁺ are infiltrating macrophages or neutrophils; and D-CD45^intCD11b⁺, microglial cells. Post-sort analysis indicated that the separated populations were >93% positive for their associated surface markers. (b) Total RNA extracted from each of 37 the separated populations (A through D) at 30 d p.i was analyzed by real-time RT-PCR for IFN-γ expression. Relative transcript levels in cells isolated from brain tissues of five animals normalized to the housekeeping gene, HPRT, expression are presented.

Performance of HSV-1-infected mice at 30 d p.i and age-matched uninfected controls were tested on a Morris water maze designed to measure spatial memory formation. Average latency associated with finding an invisible platform based on visual cues was measured daily for 12 d, with each animal receiving 4 trials/d from the same quadrant of the maze. Uninfected Balb/c (open circles) mice demonstrated lower latency rates than HSV-1 infected animals, indicating a severe spatial memory defect. A visual probe test was used to eliminate animals that could not find a visible platform after 4 trials. Data presented are mean latency (± SEM) from 6 uninfected animals and 10 HSV-1 infected animals with no visual deficits (** p<0.001).