Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Proc IEEE Int Symp Biomed Imaging. 2009;5193251:1111-1114.

MOVING BEYOND COLOR: THE CASE FOR MULTISPECTRAL IMAGING IN BRIGHTFIELD PATHOLOGY.

Author information

  • 1Center for Biomedical Imaging and Informatics, The Cancer Institute of NJ, UMDNJ-RWJMS, New Brunswick, New Jersey 08903.

Abstract

A multispectral camera is capable of imaging a histologic slide at narrow bandwidths over the range of the visible spectrum. While several uses for multispectral imaging (MSI) have been demonstrated in pathology [1, 2], there is no unified consensus over when and how MSI might benefit automated analysis [3, 4]. In this work, we use a linear-algebra framework to investigate the relationship between the spectral image and its standard-image counterpart. The multispectral "cube" is treated as an extension of a traditional image in a high-dimensional color space. The concept of metamers is introduced and used to derive regions of the visible spectrum where MSI may provide an advantage. Furthermore, histological stains which are amenable to analysis by MSI are reported. We show the Commission internationale de l'éclairage (CIE) 1931 transformation from spectrum to color is non-neighborhood preserving. Empirical results are demonstrated on multispectral images of peripheral blood smears.

PMID:
19997528
[PubMed]
PMCID:
PMC2789468
Free PMC Article

Images from this publication.See all images (5)Free text

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for PubMed Central
    Write to the Help Desk