All-fiber CARS microscopy of live cells

Opt Express. 2009 Nov 9;17(23):20700-6. doi: 10.1364/OE.17.020700.

Abstract

Using an all-fiber laser system consisting of a femtosecond Er/Yb fiber oscillator as the pump and an ultra-highly nonlinear fiber for Stokes generation, we demonstrate multimodal (TPF+SHG+CARS) non-linear optical microscopy of both tissue samples and live cells. Multimodal imaging was successfully performed with pixel dwell times as short as 4 micros at low laser powers (< 40 mW total).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Equipment Design
  • Erbium / chemistry
  • Fiber Optic Technology
  • Humans
  • Lasers
  • Microscopy / instrumentation*
  • Microscopy / methods*
  • Optics and Photonics
  • Oscillometry / methods
  • Rabbits
  • Spectrum Analysis, Raman / methods*
  • Ytterbium / chemistry

Substances

  • Erbium
  • Ytterbium