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Mol Cell Biol. 2010 Feb;30(4):935-47. doi: 10.1128/MCB.01379-09. Epub 2009 Dec 7.

Structure-specific recognition protein 1 facilitates microtubule growth and bundling required for mitosis.

Author information

  • 1Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, MS-0051, 635 Barnhill Drive, Indianapolis, IN 46202, USA.

Abstract

Tight regulation of microtubule (MT) dynamics is essential for proper chromosome movement during mitosis. Here we show, using mammalian cells, that structure-specific recognition protein 1 (SSRP1) is a novel regulator of MT dynamics. SSRP1 colocalizes with the spindle and midbody MTs, and associates with MTs both in vitro and in vivo. Purified SSRP1 facilitates tubulin polymerization and MT bundling in vitro. Knockdown of SSRP1 inhibits the growth of MTs and leads to disorganized spindle structures, reduction of K-fibers and midbody fibers, disrupted chromosome movement, and attenuated cytokinesis in vivo. These results demonstrate that SSRP1 is crucial for MT growth and spindle assembly during mitosis.

PMID:
19995907
[PubMed - indexed for MEDLINE]
PMCID:
PMC2815565
Free PMC Article
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